Yuchao LI1,2, Yuli KOU2,3, Zhao YANSONG2
1School of Chemistry and Chemical Engineering,Shandong University of Technology;, ZiBo, China
2Department of Safety, Chemistry and Biomedical Laboratory Sciences, Faculty of Engineering and Science, Western Norway University of Applied Sciences, Bergen, Norway
3Key Laboratory of Oil and Gas Fine Chemicals, Ministry of Education & Xinjiang Uygur Autonomous Region,Xinjiang University, Urumqi, China
Liquid water is an important solvent for carbon-based life, due to its special structure and hydrogen bond engaging the biochemical reactions and energy transformations in the cell. However, the liquid window of water is small within 0-100 oC at 1 atm. The hydrogen bond is the reason that water is highly adhesive and has a high heat of vaporization, high specific heat capacity and excellent solvents. These properties make water an excellent solvent that can dissolve a wind range of compounds to allow the biochemical processes to occur and the high heat capacity allows for the regulating temperature of bodies. IL exhibited a wide liquid range and also presented the hydrogen bond, indicating that the IL may also play the same role to cultivate life. When water is partly replaced using ILs to transfer energy and nutrition, life activities may happen at a wide temperature range and a wide pressure range on earth, in the solar system and the universe. The aqueous Gly-based ILs were employed as a culture medium for the cultivation of algae. Four different kinds of Gly-based ILs ([Gly]3PO4, [Gly]H2PO4, [Gly]Cl and [Gly]BF4) were first synthesized by the simple neutral reaction. The structure of synthesized ILs was confirmed by FT-IR. Further, the effect of the Gly-based ILs concentration was investigated. The growth rate of algae was tested by microscope and the UV-vis according to the absorption of chlorophyll (Figure 1). The Gly-based IL presented higher biological compatibility of algae than previous work.1 The highest concentration of glycine phosphate suit for algae cultivation is 15% and the algae could reproduce for 30 days without obvious inhibition.2 The suit concentration of [Gly]BF4 for the algae reproduction is lower than other ILs. This may be due that the [BF4]- is more toxic than the monatomic anions chloride. This research is promising work in the context of the cultivation of algae in amino acid IL based culture medium and exploring the life activity restricted zone on earth.
1 Yansong Zhao, and Yinpeng Zhen. Looking for life activity in ionic liquids. Journal of Molecular Liquids, 2022, 349: 118185.
2 Yuchao Li, Yuli Kou, Yansong Zhao, Algae cultivation in Ultra-high concentration Amino acid based ionic liquids, Journal of Molecular Liquids, submitted.